Question 1

Which of the following might lead to the generation of several incorrect PCR products?

Accepted Answer

A)   An annealing temperature that is too low
B)   An annealing temperature that is too high
C)   Performing 30 cycles of PCR
D)   Using the correct primers
E)   Including an extension stepF) D) and E)
G) A) and B)

Question 2

A recombinant DNA molecule is produced artificially and contains sequences ________.

Accepted Answer

A)   from bacteria only
B)   from yeast only
C)   from unrelated organisms
D)   from cells that have been chemically modified
E)   from prokaryotic genesF) D) and E)
G) A) and B)

Question 3

Which of the following techniques is most efficient for transforming bacterial cells?

Accepted Answer

A)   Gene gun
B)   ES cell transfer
C)   Electroporation
D)   Pronuclear microinjection
E)   Sperm-mediated transferF) A) and E)
G) B) and C)

Question 4

Explain the meaning and utility of RNA interference.

Accepted Answer

RNA interference (RNAi) is a p...

Question 5

Which of the following techniques is most commonly used to separate and analyze DNA by size?

Accepted Answer

A)   PCR
B)   Agarose gel electrophoresis
C)   DNA microarray analysis
D)   Hybridization
E)   DNA librariesF) C) and D)
G) D) and E)

Question 6

Which of the following techniques is the best choice for amplifying DNA?

Accepted Answer

A)   Southern blot analysis
B)   PCR
C)   DNA sequencing
D)   Affinity chromatography
E)   Microarray analysisF) A) and B)
G) B) and C)

Question 7

Which of the following statements about the human genome is INCORRECT?

Accepted Answer

A)   There are approximately 3.1 billion bp in the human genome.
B)   Human share a majority of genes with other species.
C)   Less than 2 percent of the genome codes for proteins.
D)   The human genome contains approximately 100,000 genes.
E)   The functions of most human genes are still unknown.F) C) and D)
G) B) and C)

Question 8

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp)  from the 5' end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce ________.

Accepted Answer

A)   one DNA fragment, 1,000 bp long
B)   three DNA fragments, two of them 300 bp long and one 400 bp long
C)   two DNA fragments, one 300 bp long and one 700 bp long
D)   two fragments, each 500 bp long
E)   none of the aboveF) B) and E)
G) A) and E)

Question 9

Imagine you wanted to use a human genomic DNA library to clone the human gene for insulin. You will be using the rat insulin gene sequence as your DNA probe. In what order would you perform the following steps to accomplish this goal? 1. Use autoradiography to identify colonies containing DNA that hybridized to the probe
2)  Grow transformed cells on media with antibiotics and X-gal for blue-white screening
3)  Ligate genomic DNA and vector DNA
4)  Cut genomic DNA and vector DNA with restriction enzymes
5)  Hybridize library DNA with labeled probe for the rat insulin gene
6)  Transform bacteria with recombinant plasmid

Accepted Answer

A)   2, 3, 6, 5, 4, 1
B)   1, 2, 5, 6, 4, 3
C)   4, 3, 6, 2, 5, 1
D)   4, 3, 5, 2, 6, 1
E)   2, 6, 5, 3, 1, 4F) C) and D)
G) B) and E)

Question 10

Dideoxyribonucleotides (ddNTPs)  used for DNA sequencing lack oxygen atoms at ________.

Accepted Answer

A)   the 1' carbon of the pentose sugar
B)   the 1' and 2' carbons of the pentose sugar
C)   the 2' and 3' carbons of the pentose sugar
D)   the 3' and 5' carbons of the pentose sugar
E)   the 5' carbon of the pentose sugarF) C) and D)
G) All of the above

Exam 3: Recombinant Dna Technology and Genomics

Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at ________.

Correct Answer
verified

Correct Answer
verified

A recombinant DNA molecule is produced artificially and contains sequences ________.

Correct Answer
verified

Which of the following techniques is the best choice for amplifying DNA?

Correct Answer
verified

Which of the following techniques is most commonly used to separate and analyze DNA by size?

Correct Answer
verified

Which of the following statements about the human genome is INCORRECT?

Correct Answer
verified

Which of the following might lead to the generation of several incorrect PCR products?

Correct Answer
verified

Which of the following techniques is most efficient for transforming bacterial cells?

Correct Answer
verified

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce ________.

Correct Answer
verified

Explain the meaning and utility of RNA interference.

Correct Answer
verified
RNA interference (RNAi) is a p...
View Answer

Exam 3: Recombinant Dna Technology and Genomics

Dideoxyribonucleotides (ddNTPs) used for DNA sequencing lack oxygen atoms at ________.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

A recombinant DNA molecule is produced artificially and contains sequences ________.

Correct AnswerverifiedShow Answer

Which of the following techniques is the best choice for amplifying DNA?

Correct AnswerverifiedShow Answer

Which of the following techniques is most commonly used to separate and analyze DNA by size?

Correct AnswerverifiedShow Answer

Which of the following statements about the human genome is INCORRECT?

Correct AnswerverifiedShow Answer

Which of the following might lead to the generation of several incorrect PCR products?

Correct AnswerverifiedShow Answer

Which of the following techniques is most efficient for transforming bacterial cells?

Correct AnswerverifiedShow Answer

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp) from the 5' end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce ________.

Correct AnswerverifiedShow Answer

Explain the meaning and utility of RNA interference.

Correct AnswerverifiedRNA interference (RNAi) is a p...View AnswerShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
RNA interference (RNAi) is a p...
View Answer